Use of chimeras in studying sterility of X-autosome translocation male mice
Journal Article
·
· Genetics; (United States)
OSTI ID:5373882
In at least 14 translocations that involve the X chromosome and one of 7 different autosomes of the mouse, heterozygous males are totally sterile. In order to help elucidate the cause of sterility, we have made aggregation chimeras between translocation and normal males. Since it is known that male phenotypes can result also from female-male chimeras, sex-linked markers as well as autosomal color markers were included to the crosses, in the hope of being able to identify the male-male type having a translocation component. While 24 were phenotypically chimeric with all 8 possible combinations represented. Cytological analysis of many of these 24 verified the diagnosis made on the basis of phenotype. Breeding tests of 74 males derived from aggregated morulae yielded 10 steriles, including 4 that were aggregates of a translocation male and nontranslocation female. However, the only two male-male chimeras with a translocation component were fertile, breeding from the normal component only. This indicates that the sterility of T(X;A) males is not due to organismic causes, such as might come about as a result of position effects. In keeping with this conclusion is the finding that in all 11 T(X;A) stocks studied at Oak Ridge, a spermatogenic block occurs at (or near) pachytene in hetrozygous males.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5373882
- Journal Information:
- Genetics; (United States), Journal Name: Genetics; (United States) Vol. 91:4; ISSN GENTA
- Country of Publication:
- United States
- Language:
- English
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