Inhibition of human lymphocyte E-rosette formation by oxygenated sterols

Journal Article · Sat Dec 01 04:00:00 EST 1979 · J. Immunol.; (United States)

OSTI ID:5372901

Streuli, R A; Chung, J; Scanu, A M; Yachnin, S

25-Hydroxycholesterol, 20..cap alpha..-hydroxycholesterol, 7..cap alpha..-hydroxycholesterol, and 5..cap alpha..-hydroxy-6-ketocholestanol, when added to cultures of human lymphocytes in lipoprotein-depleted medium (LPDM) at a concentration of 2.5 x 10/sup -5/ M, inhibit E-rosette formation with sheep red blood cells. 20..cap alpha..-Hydroxycholesterol, 7..cap alpha..-hydroxycholesterol, and 5..cap alpha..-hydroxy-6-ketocholestanol are more potent inhibitors than 25-hydroxycholesterol. The inhibitory effect of 5..cap alpha..-hydroxy-6-ketocholestanol on E-rosette formation appears after 15 min of exposure; with the other three compounds, an exposure time of 18 h is necessary. The inhibitory effect on E-rosette formation can be abolished by addition of free cholesterol, low-density lipoprotein, or high-density lipoprotein to the LPDM or by incubation of the cells in normal AB serum, but not by the addition of mevalonic acid to the LPDM. These observations suggest that the capacity of oxygenated sterol compounds (OSC) to inhibit E-rosette formation is independent of their inhibitory effect on sterol synthesis. It is possible that OSC inhibit E-rosette formation as a consequence of their insertion into the lymphocyte membrane as cholesterol analogues.

• Research Organization: Franklin McLean Memorial Research Inst., Chicago, IL
• OSTI ID: 5372901
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 123:6; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHOLESTEROL
CONNECTIVE TISSUE CELLS
HYDROXY ACIDS
HYDROXY COMPOUNDS
IMMUNE REACTIONS
INHIBITION
LEUKOCYTES
LIPIDS
LIPOPROTEINS
LYMPHOCYTES
MATERIALS
MEMBRANES
MEVALONIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
SOMATIC CELLS
STEROIDS
STEROLS