Screening for mutagenic response of six coded chemicals in the CHO/HGPRT system
A set of stringent conditions required for quantifying mutation-induction to 6-thioguanine resistance, which selects for > 98% variants with altered hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity in a near-diploid Chinese hamster ovary (CHO) cell line have been defined. The system fulfills criteria for a specific locus mutational assay and is useful in studies of mechanisms of mammalian cell mutagenesis, determination of the mutagenicity of various physical and chemical agents, and screening for mutagenic activity of some industrial chemicals. Preliminary findings on the application of this system in screening for the mutagenic activity of 6 coded chemicals supplied by the International Program for the Evaluation of Short-Term Test for Carcinogenicity are presented. The study limits itself to concerns more with mutagenic response than quantitative mutagenesis. Although our limited study of 6 out of 42 chemicals in no way could be used for evaluating the performance of the CHO/HGPRT system; nevertheless, it could shed some light on identifying certain considerations necessary for applying this system for screening the mutagenic activity of unknown chemicals.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE Office of Health and Environmental Research; US Environmental Protection Agency
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5365680
- Report Number(s):
- CONF-7910146--1
- Country of Publication:
- United States
- Language:
- English
Similar Records
CHO/HGPRT mutagenicity assay. I. Experimental procedure
Mutagenicity and cytotoxicity of ethylene oxide in the CHO/HGPRT system
Related Subjects
550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
CELL CULTURES
DATA
DATA FORMS
ENZYME ACTIVITY
ENZYMES
EXPERIMENTAL DATA
GENETIC EFFECTS
INFORMATION
ISOLATED VALUES
MUTAGEN SCREENING
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
PERFORMANCE TESTING
PHOSPHOTRANSFERASES
SCREENING
TESTING
TOXICITY
TRANSFERASES