Aphidicolin inhibits repair of DNA in UV-irradiated human fibroblasts
- Univ. of Tennessee, Oak Ridge
Aphidicolin, a specific inhibitor of DNA polymerase ..cap alpha.., is shown to inhibit DNA repair in human diploid fibroblasts. Although aphidicolin has no apparent effect on the DNA of unirradiated cells, it causes a large number of strand breaks to accumulate in UV-irradiated cellular DNA. The number of breaks is the same as the number observed following a similar dose of ultraviolet light when cells are treated with arabinofuranosyl cytosine (araC) and hydroxyurea (HU), known inhibitors of repair. Moreover, two-dimensional paper chromatography shows that aphidicolin completely blocks removal of pyrimidine dimers. These observations are discussed in light of the proposed roles of DNA polymerases ..cap alpha.. and ..beta.. in DNA replication and repair and the action of aphidicolin on polymerase ..cap alpha...
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5363586
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 99:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL REPAIR
ENZYME INHIBITORS
BIOLOGICAL EFFECTS
POLYMERASES
BIOLOGICAL FUNCTIONS
ANIMAL CELLS
CHROMATOGRAPHY
EXTERNAL IRRADIATION
FIBROBLASTS
INHIBITION
STRAND BREAKS
ULTRAVIOLET RADIATION
BIOLOGICAL RECOVERY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYMES
FUNCTIONS
IRRADIATION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
RADIATIONS
RECOVERY
REPAIR
SEPARATION PROCESSES
SOMATIC CELLS
TRANSFERASES
560121* - Radiation Effects on Cells- External Source- (-1987)