Aphidicolin inhibits repair of DNA in UV-irradiated human fibroblasts

Journal Article · Thu Apr 30 00:00:00 EDT 1981 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(81)90730-0· OSTI ID:5363586

Snyder, R D ^[1]; Regan, J D

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1. Univ. of Tennessee, Oak Ridge

Aphidicolin, a specific inhibitor of DNA polymerase ..cap alpha.., is shown to inhibit DNA repair in human diploid fibroblasts. Although aphidicolin has no apparent effect on the DNA of unirradiated cells, it causes a large number of strand breaks to accumulate in UV-irradiated cellular DNA. The number of breaks is the same as the number observed following a similar dose of ultraviolet light when cells are treated with arabinofuranosyl cytosine (araC) and hydroxyurea (HU), known inhibitors of repair. Moreover, two-dimensional paper chromatography shows that aphidicolin completely blocks removal of pyrimidine dimers. These observations are discussed in light of the proposed roles of DNA polymerases ..cap alpha.. and ..beta.. in DNA replication and repair and the action of aphidicolin on polymerase ..cap alpha...

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5363586
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 99:4; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
EXTERNAL IRRADIATION
FIBROBLASTS
FUNCTIONS
INHIBITION
IRRADIATION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATIONS
RECOVERY
REPAIR
SEPARATION PROCESSES
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES
ULTRAVIOLET RADIATION