Aphidicolin: an inhibitor of DNA repair in human fibroblasts

Journal Article · Wed Dec 31 23:00:00 EST 1980 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/2.8.795· OSTI ID:5606948

Waters, R

Aphidicolin is a specific inhibitor of DNA polymerase alpha. Its influence of DNA repair has been studied in both normal and excision deficient xeroderma pigmentosum cells exposed to u.v. irradiation at 254 nm. Single strand DNA breaks accumulated in u.v. irradiated normal cells when the inhibitor was present. Such breaks were absent in both unirradiated normal cells and in u.v. irradiated excision efficient cells incubated with the compound. The data therefore indicate that aphidicolin prevents the rejoining of single strand breaks formed during the excision repair process and imply that DNA polymerase alpha is involved in the repair of DNA in human cells.

• Research Organization: Department of Genetics, University College, Swansea, UK
• OSTI ID: 5606948
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 2:8; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
EXTREME ULTRAVIOLET RADIATION
INHIBITION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
STRAND BREAKS
TERPENES
TRANSFERASES
ULTRAVIOLET RADIATION
XP CELLS