Comparison of nucleoid and alkaline sucrose gradients in the analysis of inhibitors of DNA repair in human fibroblasts
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
In ultraviolet-irradiated human fibroblasts inhibitors of DNA polymerases alpha (cytosine arabinoside, aphidicolin) and beta (dideoxythymidine) blocked polymerization of repair patches and accumulated large numbers of single-strand breaks in alkaline sucrose (up to 4-5 in 10/sup 8/ daltons). In nucleoid gradents, however, the restoration of DNA supercoiling to control levels after relaxation during excision repair was prevented only by aphidicolin, not be cytosine arabinoside or dideoxythymidine. These chain-terminating inhibitors must therefore generate breaks in DNA that are masked by proteins (possibly repair enzymes) that cannot be removed by the high-salt treatment of nucleoid gradients. Alkaline sucrose and nucleoid gradients therefore reveal different facets of the DNA breakage and rejoining during repair. The inhibitor of poly(ADP-ribose) synthesis, 3-aminobenzamide, had no effect on nucleoid sedimentation, implying that synthesis of this polymer plays no significant role in completion of repair of ultraviolet damage in human fibroblasts.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 6123904
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 107:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Aphidicolin inhibits repair of DNA in UV-irradiated human fibroblasts
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Thu Apr 30 00:00:00 EDT 1981
· Biochem. Biophys. Res. Commun.; (United States)
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OSTI ID:5363586
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Mon Feb 29 23:00:00 EST 1988
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·
OSTI ID:7025290
Related Subjects
550200* -- Biochemistry
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
MAMMALS
MAN
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES
560111 -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
ENZYME INHIBITORS
ENZYMES
FIBROBLASTS
MAMMALS
MAN
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
STRAND BREAKS
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES