Cellular proliferation after experimental glaucoma filtration surgery
We used light microscopic autoradiography to determine the time course of cellular incorporation of tritiated thymidine (a correlate of cell division) following glaucoma filtration surgery in seven eyes of four cynomolgus monkeys with experimental glaucoma. Incorporation of tritiated thymidine was detected as early as 24 hours postoperatively. Peak incorporation occurred five days postoperatively and had returned to baseline levels by day 11. Cells incorporating tritiated thymidine included keratocytes, episcleral cells, corneal and capillary endothelial cells, and conjunctival and corneal epithelial cells. Transmission electron microscopy was correlated with the autoradiographic results to demonstrate that fibroblasts were dividing on the corneoscleral margin. These findings have potential clinical implications for the use of antiproliferative agents after filtration surgery.
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD
- OSTI ID:
- 5359488
- Journal Information:
- Arch. Ophthalmol. (Chicago); (United States), Journal Name: Arch. Ophthalmol. (Chicago); (United States) Vol. 106:1; ISSN AROPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AUTORADIOGRAPHY
AZINES
BODY
BODY AREAS
CELL PROLIFERATION
CONJUNCTIVA
CONNECTIVE TISSUE CELLS
CORNEA
DISEASES
ELECTRON MICROSCOPY
ENDOTHELIUM
EYES
FACE
FIBROBLASTS
FILTRATION
HEAD
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MEDICINE
MEMBRANES
MICROSCOPY
MONKEYS
MUCOUS MEMBRANES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
PYRIMIDINES
RIBOSIDES
SENSE ORGANS
SENSE ORGANS DISEASES
SEPARATION PROCESSES
SOMATIC CELLS
SURGERY
THYMIDINE
TISSUES
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
VERTEBRATES