Trace element distribution in the rat cerebellum
Conference
·
OSTI ID:5357713
Spatial distributions and concentrations of trace elements (TE) in the brain are important because TE perform catalytic structural functions in enzymes which regulate brain function and development. We have investigated the distributions of TE in rat cerebellum. Structures were sectioned and analyzed by the Synchrotron Radiation Induced X-ray Emission (SRIXE) method using the NSLS X-26 white-light microprobe facility. Advantages important for TE analysis of biological specimens with x-ray microscopy include short time of measurement, high brightness and flux, good spatial resolution, multielemental detection, good sensitivity, and non-destructive irradiation. Trace elements were measured in thin rat brain sections of 20-micrometers thickness. The analyses were performed on sample volumes as small as 0.2 nl with Minimum Detectable Limits (MDL) of 50 ppb wet weight for Fe, 100 ppb wet weight for Cu, and Zn, and 1 ppM wet weight for Pb. The distribution of TE in the molecular cell layer, granule cell layer and fiber tract of rat cerebella was investigated. Both point analyses and two-dimensional semi-quantitative mapping of the TE distribution in a section were used.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- Sponsoring Organization:
- DOE/ER; DOHHS
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 5357713
- Report Number(s):
- BNL-43097; CONF-8908165--2; ON: DE90003965; CNN: P41RR01838; ES04040
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
BRAIN
BREMSSTRAHLUNG
CENTRAL NERVOUS SYSTEM
CEREBELLUM
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ELEMENTS
IONIZING RADIATIONS
MAMMALS
NERVOUS SYSTEM
ORGANS
RADIATIONS
RATS
RODENTS
SPATIAL DISTRIBUTION
SYNCHROTRON RADIATION
TRACE AMOUNTS
VERTEBRATES
X RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BODY
BRAIN
BREMSSTRAHLUNG
CENTRAL NERVOUS SYSTEM
CEREBELLUM
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ELEMENTS
IONIZING RADIATIONS
MAMMALS
NERVOUS SYSTEM
ORGANS
RADIATIONS
RATS
RODENTS
SPATIAL DISTRIBUTION
SYNCHROTRON RADIATION
TRACE AMOUNTS
VERTEBRATES
X RADIATION