Molecular cloning of cDNAs of human liver and placenta NADH-cytochrome b/sub 5/ reductase
A cDNA coding for human liver NADH-cytochrome b/sub 5/ reductase was cloned from a human liver cDNA library constructed in phage lambdagt11. The library was screened by using an affinity-purified rabbit antibody against NADH-cytochrome b/sub 5/ reductase of human erythrocytes. A cDNA about 1.3 kilobase pairs long was isolated. By using the cDNA as a probe, another cDNA (pb/sub 5/R141) of 1817 base pairs was isolated that hybridized with a synthetic oligonucleotide encoding Pro-Asp-Ile-Lys-Tyr-Pro, derived from the amino acid sequence at the amino-terminal region of the enzyme from human erythrocytes. Furthermore, by using the pb/sub 5/R141 as a probe, cDNA clones having more 5' sequence were isolated from a human placenta cDNA library. The amino acid sequences deduced from the nucleotide sequences of these cDNA clones overlapped each other and consisted of a sequence that completely coincides with that of human erythrocytes and a sequence of 19 amino acid residues extended at the amino-terminal side. The latter sequence closely resembles that of the membrane-binding domain of steer liver microsomal enzyme
- Research Organization:
- Medical College of Oita, Japan
- OSTI ID:
- 5356556
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:11
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LABELLED COMPOUNDS
HYBRIDIZATION
NUCLEOTIDE DEHYDROGENASES
AMINO ACID SEQUENCE
DNA SEQUENCING
RECOMBINANT DNA
CYTOCHROMES
DNA-CLONING
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAN
PHOSPHORUS 32
PLACENTA
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHROMATOGRAPHY
CLONING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
ENZYMES
FETAL MEMBRANES
GLANDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOSPHORUS ISOTOPES
PIGMENTS
PRIMATES
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques