Characterization of high-affinity receptors for interleukin 5 on interleukin 5-dependent cell lines

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.7.2311· OSTI ID:5353193

Mita, Seiji; Tominaga, Akira; Hitoshi, Yasumichi; Sakamoto, Kiyoshi; Honjo, Tasuku; Akagi, Masanobu; Kikuchi, Yuji; Yamaguchi, Naoto; Takatsu, Kiyoshi ^[1]

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1. Kumamoto Univ. Medical School (Japan)

Interleukin 5 (IL-5) is a glycosylated polypeptide that acts as a key factor for B-cell growth and differentiation. The authors demonstrated previously that there are two classes (high and low affinity) of IL-5 receptors on murine chronic B-cell leukemic cells (BCL{sub 1}-B20). Treatment of surface-bound radiolabeled IL-5 with bivalent crosslinkers identified a polypeptide of M{sub r} 92,500. In this study, the authors analyzed characteristics of high-affinity IL-5 receptors on IL-5-dependent early B-cell lines (T-88 and T88-M), mouse myeloma cells (MOPC-104E), and BCL{sub 1}-B20 cells. All cell lines had two classes of IL-5 binding sites, but T88-M cells bore the highest number of high-affinity receptors. Disuccinimidyl tartarate crosslinking of {sup 35}S-labeled IL-5 to the receptors on the T88-M and lipopolysaccharide-stimulated BCL{sub 1}-B20 cells revealed two major {sup 35}S-labeled components of M{sub r} 92,500 and M{sub r} 160,000, even when the binding of {sup 35}S-labeled IL-5 was carried out under high-affinity conditions. The M{sub r} 92,500 component, but not the M{sub r} 160,000 component, was detected in the lysates of MOPC-104E and T-88 cells, both of which bore a large number of low-affinity receptors and a limited number of high-affinity receptors. The results suggest that the M{sub r} 92,500 component represents the complex of IL-5 with the low-affinity M{sub r} 46,500 receptor, whereas the high-affinity receptor consists of the M{sub r} 46,500 peptide and an additional peptide.

• OSTI ID: 5353193
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:7; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIFFERENTIATION
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TUMOR CELLS