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Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
Interleukin 1US (IL-1US ), one of two different polypeptide hormones with interleukin 1 (IL-1) biological activity, produced by activated human monocytes, is a 17.5-kDa protein. IL-1US binds specifically to a variety of cells; the cellular distribution of binding is consistent with reported biological responsiveness. In this report the authors show that two unrelated, but IL-1-responsive, cell lines, LBRM-33-1A5, a T-lymphoma line, and BALB/3T3, a fibroblast line, bind SVI-labeled IL-1US via similar plasma membrane receptor molecules. The T-lymphoma cells possess 238 +/- 16 plasma membrane receptors per cell and bind SVI-labeled IL-1US with an affinity of 3.6 +/- 0.9 x 10Z M . The IL-1 receptor has a molecular size of approx. = 79.5 kDa, as estimated by affinity cross-linking. The fibroblasts possess 4.8 +/- 0.5 x 10T IL-1 receptor per cell and bind SVI-labeled IL-1US with an affinity of 2.6 +/- 0.5 x 10Z M . The molecular size of the receptor molecule on the fibroblasts is approx. = 78 kDa. Despite the similarity in the characteristics of the ligand-receptor system on the two different cell types, the biological responses of the two cell types to IL-1US occur at IL-1US concentrations that differ by four orders of magnitude.
Research Organization:
Immunex Corp., Seattle, WA
OSTI ID:
6293972
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:4; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FIBROBLASTS
GROWTH FACTORS
HORMONES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOKINES
MATERIALS
MITOGENS
MOLECULAR STRUCTURE
MONOCYTES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SOMATIC CELLS
SYMPTOMS
TRACER TECHNIQUES
TUMOR CELLS