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Characterization of human interleukin-3 receptors on a multi-factor-dependent cell line

Journal Article · · Biochem. Biophys. Res. Commun.; (United States)
Recombinant human interleukin-3 (hIL-3) was radioiodinated by Bolton-Hunter method with maintenance of biological activity. Using /sup 125/I-hIL-3, hIL-3 receptors were characterized on a multi-factor-dependent cell line TF-1. Equilibrium binding studies revealed the existence of a single class of binding sites (667 +/- 306 sites/cell) with a Kd of 173 +/- 25 pM. Affinity labeling of TF-1 cells with /sup 125/I-IL-3 yielded two bands of 150 kDa and 85 kDa, implying molecular weights of 135 kDa and 70 kDa for the hIL-3 receptors.
Research Organization:
Univ. of Tokyo (Japan)
OSTI ID:
5934344
Journal Information:
Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 161:1; ISSN BBRCA
Country of Publication:
United States
Language:
English