Characterization of receptors for VIP on pancreatic acinar cell plasma membranes using covalent cross-linking

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5445326

McArthur, K E; Wood, C L; O'Dorisio, M S; Zhou, Z C; Gardner, J D; Jensen, R T

Vasoactive intestinal peptide (VIP) receptors on guinea pig pancreatic acini differ from those on all other tissues in containing a high-affinity VIP receptor and a low-affinity VIP receptor that has a high affinity for secretin. To characterize the molecular components of these receptors, /sup 125/I-VIP was covalently cross-linked to these receptors by four different cross-linking agents: disuccinimidyl suberate, ethylene glycol bis (succinimidyl succinate), dithiobis (succinimidylpropionate), and m-maleimidobenzoyl N-hydroxysuccinimide ester. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated a single major polypeptide band of M/sub r/ 45,000 and a minor polypeptide band of M/sub r/ 30,000 were cross-linked to /sup 125/I-VIP. Covalent cross-linking only occurred when a cross-linking agent was added, was inhibited by GTP, was inhibited by VIP receptor agonist or antagonists that interact with VIP receptors, and not by other pancreatic secretagogues that interact with difference receptors. Thus the high-affinity VIP receptor on pancreatic acinar cell membranes consists of a single major polypeptide of M/sub r/ 45,000, and this polypeptide is not a subunit of a larger disulfide-linked structure. Furthermore, either the low-affinity VIP/secretin-preferring receptor was not covalently cross-linked under the experimental conditions or it consist of a major polypeptide with the same molecular weight as the high-affinity VIP receptor.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 5445326
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:3; ISSN AJPHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENDOCRINE GLANDS
GASTROINTESTINAL TRACT
GLANDS
GUINEA PIGS
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDES
POLYMERIZATION
PROTEINS
RADIOISOTOPES
RECEPTORS
RODENTS
VERTEBRATES