Characterization of the cell surface receptor for a multi-lineage colony-stimulating factor (CSF-2. cap alpha. )
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:5179154
/sup 125/-Labeled colony-stimulating factor (CSF) 2..cap alpha.. (interleukin 3, multi-CSF, and mast cell growth factor) was used to characterize receptors specific for this lymphokine on the cell surface of the factor-dependent cell line FDC-P2. CSF-2..cap alpha.. binding to these cells was specific and saturable. Among a panel of lymphokines and growth factors, only unlabeled CSF-2..cap alpha.. was able to compete for the binding of /sup 125/I-labeled CSF-2..cap alpha.. to cells. Equilibrium binding studies revealed that CSF-2..cap alpha.. bound to 434 +/- 281 receptors/cell with a K/sub a/ of 8.7 +/- 3.9 x 10/sup 9/ M/sup -1/. Affinity cross-linking experiments with the homobifunctional cross-linking reagents disuccinimidyl suberate, disuccinimidyl tartrate, and dithiobis(succinimidyl propionate) produced a radiolabeled band of M/sub r/ = 97,000 on intact cells and in purified cell membranes, while an additional band of M/sub r/ = 138,000 was produced upon cross-linking to intact cells only. The relationship between these two bands is discussed. The results indicate that the receptor for CSF-2..cap alpha.. on FDC-P2 cells consists at a minimum of a subunit of M/sub r/ = 72,500.
- Research Organization:
- Immunex Corp., Seattle, WA
- OSTI ID:
- 5179154
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 261:1; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL MEMBRANES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GROWTH
GROWTH FACTORS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LYMPHOKINES
MAST CELLS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SOMATIC CELLS
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
CELL MEMBRANES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GROWTH
GROWTH FACTORS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LYMPHOKINES
MAST CELLS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SOMATIC CELLS
TRACER TECHNIQUES