Studies on the identification and characterization of the receptor for heparin binding growth factor 1
Thesis/Dissertation
·
OSTI ID:6325580
Receptor-binding studies using biologically active {sup 125}I-HBGF-1 revealed that many cells of mesenchymal origin possess HBGF-1 receptors with affinities ranging from 100-200 pM and 2.0 x 10{sup 3} sites per cell. The HBGF-1 receptor was identified by convalently cross-linking {sup 125}I-HBGF-1 to receptor-bearing cells with the homobifunctional reagent disuccinimidyl suberate (DSS). Cross-linking of {sup 125}I-HBGF-L to M{sub r} 150,000 and M{sub r} 130,000 cell surface polypeptides was inhibited by excess unlabeled HBGF-1 but not by other growth factors. The {sup 125}I-HBGF receptor cross-linked complex was susceptible to cleavage by cyanogen bromide and trypsin, confirming the polypeptide nature of HBGF-1 receptor. Binding studies carried at 37{degree}C and 4{degree}C demonstrated that the HBGF-1 receptor is down-regulated at 37{degree}C with internalization of {sup 125}I-HBGF-1. The degradation of internalized {sup 125}I-HBGF-1 was inhibited by the lysosomotropic agent chloroquine, suggesting that internalized {sup 125}I-HBGF-1 is directed to the lysosomal cellular compartment. The lymphokine IFN-{sub {gamma}} was shown to inhibit HBGF-1 induced proliferation of endothelial cells in a dose-dependent and reversible manner. Studies were also undertaken to determine if cells that possess HBGF-1 receptors also express the growth factor.
- Research Organization:
- George Washington Univ., Washington, DC (USA)
- OSTI ID:
- 6325580
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINES
ANIMAL TISSUES
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CELL PROLIFERATION
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEPARIN
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LYMPHOKINES
MEMBRANE PROTEINS
MITOGENS
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYMERIZATION
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SACCHARIDES
TISSUES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINES
ANIMAL TISSUES
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOHYDRATES
CELL PROLIFERATION
CHEMICAL COMPOSITION
CHEMICAL REACTIONS
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEPARIN
INHIBITION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LYMPHOKINES
MEMBRANE PROTEINS
MITOGENS
MUCOPOLYSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYMERIZATION
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SACCHARIDES
TISSUES
TRACER TECHNIQUES