In vitro phagocytosis and intracellular survival of Campylobacter jejuni with phagocytes
Thesis/Dissertation
·
OSTI ID:5348620
In vitro phagocytosis and intracellular survival of Campylobacter jejuni was studied using three types of mononuclear phagocytes: a J774G8 peritoneal macrophage line, resident BABL/c peritoneal macrophages and human peripheral blood monocytes. In phagocytosis assays using CFU determinations, phagocytosis increased steadily over an 8 hr time period. Results obtained using a /sup 51/Cr assay indicated no consistent significant difference between phagocytosis of C. jejuni between the three mononuclear phagocytes or PMN's and that maximum infection occurred prior to 0.5 hr and maintained throughout the 4 hr assay. Further investigation of the mechanism of attachment and entry of C. jejuni revealed this process required the expenditure of energy by the phagocyte, but was not inhibited by inhibitors of microfilament functions. In addition, phagocytosis was enhanced by the presence of 20% FCS,
- Research Organization:
- Chicago Medical School, IL (USA)
- OSTI ID:
- 5348620
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MACROPHAGES
MATERIALS
MICROORGANISMS
MONOCYTES
NEUTROPHILS
NUCLEI
PHAGOCYTES
PHAGOCYTOSIS
RADIOISOTOPES
SOMATIC CELLS
SURVIVAL CURVES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MACROPHAGES
MATERIALS
MICROORGANISMS
MONOCYTES
NEUTROPHILS
NUCLEI
PHAGOCYTES
PHAGOCYTOSIS
RADIOISOTOPES
SOMATIC CELLS
SURVIVAL CURVES
TRACER TECHNIQUES