Effects of endogenous antidiuretic hormone (ADH) on macrophage phagocytosis

Journal Article · Fri Jul 01 04:00:00 EDT 1983 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/31.7.6854008· OSTI ID:5394853

Fernandez-Repollet, E; Opava-Stitzer, S; Tiffany, S; Schwartz, A

Although several studies have indicated that antidiuretic hormone (ADH) enhances the phagocytic function of the reticuloendothelial system (RES) in shock syndromes, it remains unknown what influence ADH exerts upon the individual phagocytic components of this system. The present investigation was designed to evaluate the effects of endogenous ADH on the phagocytic activity of peritoneal macrophage cells. As a phagocytic stimuli, fluorescent methacrylate microbeads were injected intraperitoneally into Brattleboro (ADH deficient) and normal Long Evans rats in the presence and absence of exogenous ADH. Peritoneal cells were harvested 19-22 hr after the administration of the microbeads and the percent phagocytosis was determined in macrophage cells using a fluorescence-activated cell sorter (FACS II). Our results indicate that the percentage of peritoneal macrophages ingesting the fluorescent methacrylate microbeads was significantly reduced in the absence of ADH (Brattleboro rats: 5.4 +/- 0.6% versus Long Evans rats: 16.8 +/- 2.3%; p less than 0.001). In addition, our data demonstrate that exogenous administration of ADH significantly enhanced macrophage phagocytosis in Brattleboro (14.7 +/- 2.2%) and normal Long Evans (49.6 +/- 4.5%) rats. These data suggest, for the first time, that endogenous ADH might play a modulatory role in the phagocytic activity of a specific component of the RES, namely, the macrophage cell.

• Research Organization: Department of Pharmacology, School of Medicine, University of Puerto Rico, San Juan
• OSTI ID: 5394853
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 31:7; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL SHOCK
BODY
CONNECTIVE TISSUE CELLS
DIURETICS
DRUGS
HORMONES
MACROPHAGES
MAMMALS
PATHOLOGICAL CHANGES
PHAGOCYTES
PHAGOCYTOSIS
PHYSIOLOGY
RATS
RETICULOENDOTHELIAL SYSTEM
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES