Dual laser beam focussing for flow cytometry through a single crossed cylindrical lens pair

Journal Article · Wed Jul 01 04:00:00 EDT 1981 · Cytometry (Baltimore); (United States)

DOI:https://doi.org/10.1002/cyto.990020103· OSTI ID:5347924

Watson, J V

Two laser beams (argon 488 nm blue, and krypton UV lines) have been focussed either to the same point or to two different points in a vertical plane in a multiparameter flow cytometer. The technique exploits the focal length shortening of the longer wavelength beam that occurs with spherical and astigmatic lens aberrations. Ethidium bromide-stained DNA is excited by both the argon 488 nm and krypton UV lines and the excitation intensities of both beams were set to give the same red fluorescence pulse height from ethidium bromide-stained nuclei. DNA histograms were obtained for sequential focussing of the two beams and these were virtually identical irrespective of which beam was the first excitor and irrespective of which beam the histogram was recorded from. Coincident focussing of the two exciting beams resulted in a histogram of double the emission intensity.

• OSTI ID: 5347924
• Journal Information: Cytometry (Baltimore); (United States), Journal Name: Cytometry (Baltimore); (United States) Vol. 2:1; ISSN CYTOD
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
CELL CYCLE
CELL FLOW SYSTEMS
CELL NUCLEI
DESIGN
DNA
ELECTROMAGNETIC RADIATION
LASER RADIATION
MONOCHROMATIC RADIATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS