Primary structure of the. cap alpha. -subunit of Na/sup +/, K/sup +/-ATPase. II. Isolation, reverse transcription, and cloning of messenger RNA

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)

OSTI ID:5346128

Petrukhin, K E; Broude, N E; Arsenyan, S G; Grishin, A V; Dzhandzhugazyan, K N; Modyanov, N N

The messenger RNA coding the ..cap alpha..-subunit of Na/sup +/,K/sup +/-ATPase has been isolated from the outer medullary layer of porcine kidneys. The mRNA gives a specific hybridization band in the 25S-26S region with three oligonucleotide probes synthesized on the basis of information on the structure of three peptides isolated from a tryptic hydrolyzate of the ..cap alpha..-subunit of Na/sup +/,K/sup +/-ATPase. The translation of the mRNA in Xenopus laevis oocytes followed by immunochemical identification of the products of synthesis confirmed the presence of the mRNA of the ..cap alpha..-subunit of Na/sup +/,K/sup +/-ATPase in an enriched fraction of poly(A/sup +/)-RNA. This preparation has been used for the synthesis of cloning of double-stranded cDNA.

• Research Organization: M.M. Shemyakin Institute of Bioorganic Chemistry, Moscow (USSR)
• OSTI ID: 5346128
• Journal Information: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Journal Name: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States) Vol. 11:12; ISSN SJBCD
• Country of Publication: United States
• Language: English

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550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ACID ANHYDRASES
ALKALI METAL COMPOUNDS
ANIMALS
ANTIGENS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BODY
CARBOXYLIC ACID SALTS
CLONING
CODONS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYMES
EVEN-ODD NUCLEI
GENES
HALIDES
HALOGEN COMPOUNDS
HYBRIDIZATION
HYDROLASES
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHOHYDROLASES
PHOSPHORS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POTASSIUM COMPOUNDS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RNA
SODIUM COMPOUNDS
SODIUM IODIDES
STRUCTURAL CHEMICAL ANALYSIS
SULFUR 35
SULFUR ISOTOPES
SWINE
SYNTHESIS
TRACER TECHNIQUES
TRANSCRIPTION
VERTEBRATES