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Problems connected with the use of oligonucleotide probes with a high degree of degeneracy. Identification of mRNA and of cDNA clones corresponding to the gene of the. cap alpha. -subunit of Na/sup +/, K/sup +/-ATPase

Journal Article · · Sov. J. Bioorg. Chem. (Engl. Transl.); (United States)
OSTI ID:5280807
; ; ; ; ; ; ;
To identify and search for nucleotide sequences containing the structural part of the gene of the ..cap alpha..-subunit of Na/sup +/, K/sup +/-ATPase, 17-membered oligonucleotide probes corresponding to the peptide Lys-Asp-Ala-Phe-Gln-Asn have been synthesized. It has been shown that, with a 64-fold degeneracyd, the 17-membered probe is suitable only for the identification of a specific sequence in mRNA. To search for clones containing cDNA fragments, preliminary fractionation of the probes with the aid of HPLC or the resynthesis of groups of oligonucleotides with a lower degeneracy is necessary.
Research Organization:
M. M. Shemyakin Institute of Bioorganic Chemistry, Moscow (USSR)
OSTI ID:
5280807
Journal Information:
Sov. J. Bioorg. Chem. (Engl. Transl.); (United States), Journal Name: Sov. J. Bioorg. Chem. (Engl. Transl.); (United States) Vol. 11:12; ISSN SJBCD
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALKALI METAL COMPOUNDS
ANIMALS
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIODEGRADATION
BIOSYNTHESIS
BODY
CHEMICAL REACTIONS
CHROMATOGRAPHY
CLONING
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DNA
DNA SEQUENCING
DNA-CLONING
DOMESTIC ANIMALS
ENZYMATIC HYDROLYSIS
ENZYMES
FRACTIONATION
HYBRIDIZATION
HYDROLASES
HYDROLYSIS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
MAMMALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHOHYDROLASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POTASSIUM COMPOUNDS
PROTEINS
RADIOENZYMATIC ASSAY
RADIOISOTOPES
RNA
SEPARATION PROCESSES
SODIUM COMPOUNDS
SOLVOLYSIS
STRUCTURAL CHEMICAL ANALYSIS
SWINE
SYNTHESIS
TRANSFERASES
VERTEBRATES