Oncogene transcription in normal human IMR-90 fibroblasts: induction by serum and tetradecanoyl phorbol acetate
Journal Article
·
· Life Sci.; (United States)
The authors report studies of oncogene transcription induced by the addition of serum to quiescent cultures of human IMR-90 fibroblasts. Oncogene messenger RNAs for c-myc, c-erbB and c-ras were increased in a specific temporal sequence after the addition of serum. Compounds that are proposed to exert their actions by the stimulation of cell growth were tested for their effect on oncogene transcription in IMR-90 fibroblasts. The tumor promoter tetradecanoyl phorbol acetate (TPA) was found to selectively induce the transcription of c-myc without observable effect on the transcription of the other oncogenes studied, and without inducing cell division. The inactive analog, phorbol didecanoate (PDD), and two complete carcinogens dimethylbenzanthracene (DMBA) and 4-nitro quinoline-1-oxide (4NQO) were without effect on the transcription of the genes studied. These results suggest that the complete ordered sequence of gene transcription is necessary to achieve the physiologic response of cell division, and that classical promoters and complete carcinogens achieve their effects through different pathways.
- Research Organization:
- Thomas Jefferson Univ., Philadelphia, PA (USA)
- OSTI ID:
- 5341183
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:14; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZANTHRACENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL DIVISION
CELL TRANSFORMATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
ESTERS
FIBROBLASTS
GENES
HYDROCARBONS
MAMMALS
MAN
NITRO COMPOUNDS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHORBOL ESTERS
PRIMATES
SOMATIC CELLS
TRANSCRIPTION
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZANTHRACENE
BIOLOGICAL EFFECTS
CARCINOGENS
CELL DIVISION
CELL TRANSFORMATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
ESTERS
FIBROBLASTS
GENES
HYDROCARBONS
MAMMALS
MAN
NITRO COMPOUNDS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHORBOL ESTERS
PRIMATES
SOMATIC CELLS
TRANSCRIPTION
VERTEBRATES