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An in vitro investigation of genetic susceptibility to cancer in diploid fibroblasts from retinoblastoma patients

Journal Article · · Teratog., Carcinog., Mutagen.; (United States)
OSTI ID:5335111
; ; ;
Thirty-two diploid fibroblast strains from individuals with the three forms of retinoblastoma were examined with an in vitro clonogenic survival assay to determine their sensitivity to killing by X rays. Strains from sporadic unilateral retinoblastoma patients and normal controls were indistinguishable from one another (group D0's . 147 +/- 15 rads and 146 +/- 5 rads, respectively) while the strains from patients with the hereditary form of the disease were significantly more X-ray sensitive (D0 . 111 +/- 12). Strains derived from individuals with the D-deletion form of the disease resembled the hereditary strains with respect to sensitivity and heterogeneity, suggesting a possible, common etiology for these two forms of the disease. A DNA-repair defect is hypothesized as reflected by the observed hypersensitivity to X-irradiation in these cells. We suggest that this defect may be associated with the enhanced frequency of spontaneous and radiation-induced second tumors seen in some retinoblastoma patients.
Research Organization:
Joint Center for Radiation Therapy, Harvard Medical School, Boston
OSTI ID:
5335111
Journal Information:
Teratog., Carcinog., Mutagen.; (United States), Journal Name: Teratog., Carcinog., Mutagen.; (United States) Vol. 1:2; ISSN TCMUD
Country of Publication:
United States
Language:
English

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Related Subjects

550400 -- Genetics
550900 -- Pathology
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOASSAY
BIOLOGICAL VARIABILITY
BIOLOGY
BODY
BODY AREAS
CELL KILLING
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
EYES
FACE
FIBROBLASTS
GENETIC VARIABILITY
GENETICS
HEAD
HEREDITARY DISEASES
IN VITRO
IONIZING RADIATIONS
NEOPLASMS
ORGANS
PATHOLOGY
PATIENTS
RADIATIONS
RADIOSENSITIVITY
RETINA
SENSE ORGANS
SOMATIC CELLS
X RADIATION