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Triethyllead treatment of cultured brain cells. Effect on accumulation of radioactive precursors in galactolipids

Journal Article · · Neurochem. Res.; (United States)
DOI:https://doi.org/10.1007/BF00964836· OSTI ID:5334280
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Cultured cells from chick embryo brains were studied for their sensitivity to triethyllead. Triethyllead chloride (3.16 microM) was added to the nutrient medium and incubated for 48 hr with the cells. Morphological changes in light microscope and radioactive labeling of galactolipids were assayed. Triethyllead treatment reduced the number of neuronal cells with processes. Morphological changes were not observed in glial cells. The (/sup 35/S)sulfate labeling of sulfatides was reduced to 50%. The (/sup 3/H)serine labeling of cerebrosides with alpha-hydroxy fatty acids was not influenced, while the (/sup 3/H)serine labeling of cerebrosides with nonhydroxy fatty acids was inhibited 40% in one- and two- but not in three-week-old cultures. The results indicate that the nerve cell response to triethyllead in cultures is selective, since the neurons are more sensitive than the glia cells and the labeling of sulfatides is more sensitive than that of cerebrosides.

OSTI ID:
5334280
Journal Information:
Neurochem. Res.; (United States), Journal Name: Neurochem. Res.; (United States) Vol. 6:2; ISSN NERED
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
BRAIN
BUILDUP
CARBOHYDRATES
CELL CULTURES
CENTRAL NERVOUS SYSTEM
CEREBROSIDES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
GLYCOLIPIDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LEAD COMPOUNDS
LIGHT NUCLEI
LIPIDS
METABOLISM
MORPHOLOGY
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SENSITIVITY
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES