Differential radiosensitivity of mouse embryonic neurons and glia in cell culture. [UV and gamma radiation]

Journal Article · Sun May 01 04:00:00 EDT 1977 · J. Neuropathol. Exp. Neurol.; (United States)

DOI:https://doi.org/10.1097/00005072-197705000-00015· OSTI ID:7216531

Dambergs, R; Kidson, C

The responses of neurons and glial cells to ultraviolet and ..gamma..-radiation were studied in cell cultures of embryonic mouse brains. A decrease in the ratio of glia to neurons occurred after both forms of irradiation. (/sup 3/H)thymidine labelling followed by autoradiography revealed that all glia were capable of replication, whereas 70 percent of neurons were non-replicating under the conditions of the study. Ultraviolet radiation caused a decrease in the proportion of replicating neurons but did not affect the proportion of replicating glia, whereas ..gamma..-radiation caused a decrease in DNA replication in both cell types. Levels of ultraviolet radiation-induced unscheduled DNA synthesis were lower in neurons than in glia. It is concluded that sensitivity to both ionizing and ultraviolet radiation of neurons and glial cells in embryonic brain cultures is determined primarily by the capacity for and state of DNA replication. Neurons which have already reached the stage of terminal differentiation are more resistant than replicating neurons of glial cells.

• Research Organization: Univ. of Queensland, Brisbane
• OSTI ID: 7216531
• Journal Information: J. Neuropathol. Exp. Neurol.; (United States), Journal Name: J. Neuropathol. Exp. Neurol.; (United States) Vol. 36:3; ISSN JNENA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BODY
BRAIN
CELL CULTURES
CELL PROLIFERATION
CENTRAL NERVOUS SYSTEM
DNA
ELECTROMAGNETIC RADIATION
EMBRYOS
GAMMA RADIATION
IONIZING RADIATIONS
MAMMALS
MICE
NERVE CELLS
NERVOUS SYSTEM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RODENTS
SOMATIC CELLS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES