Effect of bleomycin on aerated and hypoxic cells in vitro, in combination with irradiation
Conference
·
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
OSTI ID:5334011
Hamster V79 cells, cultured in vitro, were treated for 4 hr at 37.5/sup 0/C with graded concentrations of bleomycin (BLM). Cells were found to be significantly more resistant to the drug under hypoxic than under aerated conditions. When misonidazole was used in combination with BLM, the survival of hypoxic cells was reduced below that for aerated cells. The cytotoxicity of BLM was observed to be greatly enhanced at elevated temperatures. Experiments in which both BLM and x-rays were used indicate additivity of effects when the 2 modalities are applied together. The cell killing potential of a combination of given doses of x-rays and BLM is greatly enhanced by a time interval between the 2 treatments of sufficient duration for the resistant survivors of the first treatment to move into a sensitive phase of the cell cycle before the second is delivered.
- Research Organization:
- Columbia Univ., New York, NY
- DOE Contract Number:
- EP-78-S-02-4733
- OSTI ID:
- 5334011
- Conference Information:
- Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 5:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLEOMYCIN
CELL CULTURES
CELL CYCLE
CELL KILLING
DATA
DATA FORMS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HAMSTERS
INFORMATION
IONIZING RADIATIONS
ISOLATED VALUES
MAMMALS
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RESPONSE MODIFYING FACTORS
RODENTS
SURVIVAL CURVES
TIME DEPENDENCE
TOXICITY
TUMOR CELLS
VERTEBRATES
X RADIATION
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANOXIA
ANTIBIOTICS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLEOMYCIN
CELL CULTURES
CELL CYCLE
CELL KILLING
DATA
DATA FORMS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HAMSTERS
INFORMATION
IONIZING RADIATIONS
ISOLATED VALUES
MAMMALS
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RESPONSE MODIFYING FACTORS
RODENTS
SURVIVAL CURVES
TIME DEPENDENCE
TOXICITY
TUMOR CELLS
VERTEBRATES
X RADIATION