Differential hypoxic cytotoxicity of bioreductive agents determined in vitro by the MTT assay
Journal Article
·
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
We obtained good agreement between the MTT assay and conventional clonogenic assays regarding the concentration and contact time required to produce a given level of killing of Chinese hamster V79 cells treated in either air of N/sub 2/ with a range of bioreductive cytotoxic drugs. All agents chosen for these experiments represented classes of compounds known to be more toxic towards hypoxic cells than they are to aerobic cells. Namely a quinone, mitomycin C; a di-N-oxide, SR4233; and a number of nitro-heterocyclics including misonidazole. The MTT assay is carried out with V79 cells attached to the bottom of 1 cm glass wells within a 24 well plate. All procedures, that is drug exposure, cell growth, metabolism of MTT are then carried out in situ. To measure optical density we used an ELIZA plate reader modified to take 24-well plates. We propose that this method provides a simple, rapid procedure for evaluating the cytotoxicity of bioreductive drugs.
- Research Organization:
- MRC Radiobiology Unit, Didcot, Oxon (England)
- OSTI ID:
- 6149150
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 16:4; ISSN IOBPD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
AZOLES
BIOASSAY
BODY
CELL KILLING
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DRUGS
ELEMENTS
FIBROBLASTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
LUNGS
MAMMALS
METABOLISM
MITOMYCIN
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXYGEN
RADIOSENSITIZERS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
THIAZOLES
TOXICITY
TRIAZINES
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AZINES
AZOLES
BIOASSAY
BODY
CELL KILLING
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DRUGS
ELEMENTS
FIBROBLASTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
LUNGS
MAMMALS
METABOLISM
MITOMYCIN
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXYGEN
RADIOSENSITIZERS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
THIAZOLES
TOXICITY
TRIAZINES
VERTEBRATES