Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus

Journal Article · Mon Mar 31 23:00:00 EST 1980 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5333528

Abshire, R L; Bain, B; Williams, T

A spore suspension model and a procedure for recovering ultraviolet (uv)-irradiated spores of Bacillus pumilus were investigated. A most-probable-number tube dilution method using double-strength Trypticase soy broth was found to be superior to the agar plate method for recovering optimal numbers of spores irradiated with sublethal doses of uv energy. Aqueous suspensions of B. pumilus survived uv doses up to 108,000 ergs/mm/sup 2/ as determined by a most-probable-number recovery and estimation procedure. Resistance and stability data were consistent and reproducible, indicating the dependability of this method for recovering uv-damaged spores. The procedures used to collect information concerning resistance characteristics for two strains of B. pumilus are discussed.

• Research Organization: Alcon Laboratories, Fort Worth, TX
• OSTI ID: 5333528
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 39:4; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACTERIA
BACTERIAL SPORES
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
IRRADIATION
MICROORGANISMS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RADIOSTERILIZATION
REPRODUCTION
SPORES
STERILIZATION
SUBLETHAL IRRADIATION
ULTRAVIOLET RADIATION