Gamma Irradiation of Bacillus subtilis Spores
When aqueous suspensions of Bacillus subtilis spores were exposed to Co⁶⁰ gamma irradiation and subsequently stored at 0 deg and 20 deg C, survival was greater at 20 deg than at 0 deg . Slopes of the lines relating dose to numbers of survivors were not parallel, but converged at approximates total survival at 0 dose of radiation. Thus spores subsequentiy stored at 20 deg survived larger doses of radiation than the spores subsequently stored at 0 deg . When an aqueous suspension of B.. subtilis spores was heated at 60 deg C for 3 min, 60 deg for 15 min, or 90 deg for 5 min, there was a progressive increase in the number of spores which produce colonies on agar plates. But if a second sample of the spore suspension was first irradiated and then heated in the same way, there was a decrease in the number of spores producing colonies on agar plates. The results indicate that pre-irradiation sensitizes B. subtilis spores to a degree of heating normally sublethal and used to activate dormant spore suspensions. (H.H.D.)
- Research Organization:
- London Univ.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-033687
- OSTI ID:
- 4684179
- Journal Information:
- Journal of Pharmacy and Pharmacology, Journal Name: Journal of Pharmacy and Pharmacology Journal Issue: 1 Vol. 15; ISSN 0022-3573
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
Similar Records
Thermorestoration of mutagenic radiation damage in bacterial spores. [Bacillus subtilis, electrons]
EFFECT OF RADIATION ENVIRONMENT ON THE THERMAL RESISTANCE OF IRRADIATED SPORES OF BACILLUS SUBTILIS