Cellulose degradation and monitoring of viscosity decrease in cultures of Cellulomonas uda grown on printed newspaper

Journal Article · Mon Oct 01 04:00:00 EDT 1984 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260261005· OSTI ID:5330212

Rapp, P; Reng, H; Hempel, D M; Wagner, F

The rheological behavior of cultures of Cellulomonas uda with shredded printed newspaper as the carbon source was studied. The initial substrate concentrations ranged from 23 to 60 g/l. The changes in apparent viscosity were followed on-line by applying a commercially available process viscometer and discretely using a rotational viscometer with an anchor impeller. During the time of highest cellulose degradation, the broths exhibited a pseudoplastic behaviour which could be explained satisfactorily by the power-law model. At the end of cultivation when cellulose degradation slowed down, the broths became Newtonian in behaviour. Endo-1, 4-..beta..-glucanase, 1,4-..beta..-xylanase, ..beta..-glucosidase, and ..beta..-xylosidase activities were also determined during cultivation as well as cellulose degradation and cell mass production. The beginning of endoglucanase formation and the start of the final viscosity decrease of the bacterial paper pulp suspensions could be correlated.

• Research Organization: Gesellschaft fuer Biotechnologische Forschung MbH, Braunschweig, Germany, F.R.
• OSTI ID: 5330212
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 26:10; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
BACTERIA
BIODEGRADATION
CARBOHYDRATES
CELL CULTURES
CELLULASE
CELLULOLYTIC ACTIVITY
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
GLUCOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PAPER
POLYSACCHARIDES
RHEOLOGY
SACCHARIDES
VISCOSITY
XYLANASE