Inhibition of cellulolytic activity by lactate in a Cellulomonas uda species

Journal Article · Mon Apr 20 00:00:00 EDT 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260290616· OSTI ID:6204533

Chosson, J

According to Stackebrandt and Kandler, Cellulomonas are aerobic, mesophilic, nonsporeforming, cellulose-utilizing bacteria which produce acid from glucose. Bacteria belonging to this genus secrete three types of enzyme involved in cellulolysis: endoglucanases, exoglucanases, and ..beta..-glucosidases or cellobiose phosphorylases. Inhibition of cellulolytic activity by glucose and cellobiose was evidenced in several Cellulomonas species. Particularly, Stoppok and coworkers reported in Cellulomonas uda a complete repression of endoglucanases synthesis by 4.8 and 2.7 g/l glucose and cellobiose, respectively. In addition, 9 g/l glucose had no effect on the endoglucanase activity, but cellobiose at the same concentration reduced it by 50%. During batch culture of C. uda on cellulose (20 g/l), degradation of the polymer remained incomplete in spite of very low concentrations of reducing sugars (0.4 g/l) in the culture supernatant. Furthermore, a careful analysis of organic acids evidenced an accumulation of L(+)-lactate up to 0.8 g/l. In this communication, the author reports the inhibitory effect of lactate on the cellulolytic activity of C. uda strains JC 3. 12 references.

• Research Organization: Institut National de la Recherche Agronomique, Cedex, France
• OSTI ID: 6204533
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 29:6
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELLULOLYTIC ACTIVITY
INHIBITION
AEROBIC CONDITIONS
BATCH CULTURE
CELLOBIOSE
CELLULOSE
ENZYME ACTIVITY
GLUCOSE
LACTATES
MESOPHILIC CONDITIONS
ALDEHYDES
CARBOHYDRATES
CARBOXYLIC ACID SALTS
DISACCHARIDES
HEXOSES
MICROORGANISMS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
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