Inhibition of cellulolytic activity by lactate in a Cellulomonas uda species

Journal Article · Mon Apr 20 04:00:00 EDT 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260290616· OSTI ID:6204533

Chosson, J

According to Stackebrandt and Kandler, Cellulomonas are aerobic, mesophilic, nonsporeforming, cellulose-utilizing bacteria which produce acid from glucose. Bacteria belonging to this genus secrete three types of enzyme involved in cellulolysis: endoglucanases, exoglucanases, and ..beta..-glucosidases or cellobiose phosphorylases. Inhibition of cellulolytic activity by glucose and cellobiose was evidenced in several Cellulomonas species. Particularly, Stoppok and coworkers reported in Cellulomonas uda a complete repression of endoglucanases synthesis by 4.8 and 2.7 g/l glucose and cellobiose, respectively. In addition, 9 g/l glucose had no effect on the endoglucanase activity, but cellobiose at the same concentration reduced it by 50%. During batch culture of C. uda on cellulose (20 g/l), degradation of the polymer remained incomplete in spite of very low concentrations of reducing sugars (0.4 g/l) in the culture supernatant. Furthermore, a careful analysis of organic acids evidenced an accumulation of L(+)-lactate up to 0.8 g/l. In this communication, the author reports the inhibitory effect of lactate on the cellulolytic activity of C. uda strains JC 3. 12 references.

• Research Organization: Institut National de la Recherche Agronomique, Cedex, France
• OSTI ID: 6204533
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 29:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AEROBIC CONDITIONS
ALDEHYDES
BACTERIA
BATCH CULTURE
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CELLOBIOSE
CELLULOLYTIC ACTIVITY
CELLULOSE
DISACCHARIDES
ENZYME ACTIVITY
GLUCOSE
HEXOSES
INHIBITION
LACTATES
MESOPHILIC CONDITIONS
MICROORGANISMS
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES