Production and properties of xylan-degrading enzymes from Cellulomonas uda

Journal Article · Tue Apr 01 04:00:00 EST 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5328232

Rapp, P; Wagner, F

Xylan degradation and production of ..beta..-xylanase and ..beta..-xylosidase activities were studied in cultures of Cellulomonas uda grown on purified xylan from birchwood. Beta-xylanase activity was found to be associated with the cells, although in various degrees. The formation of ..beta..-xylanase activity was induced by xylotriose and repressed by xylose. Beta-xylosidase activity was cell bound. Both constitutive and inducible ..beta..-xylosidase activities were suggested. Beta-xylanase and ..beta..-xylosidase activities were inhibited competitively be xylose. Beta-xylanase activity had a pronounced optimum pH of 5.8, whereas the optimum pH of ..beta..-xylosidase activity ranged from 5.4 to 6.1. The major products of xylan degradation by a crude preparation of ..beta..-xylanase activity, in decreasing order of amount, were xylobiose, xylotriose, xylose and small amounts of xylotetraose. This pattern suggests that ..beta..-xylanase activity secreted by C. uda is of the endosplitting type. Supernatants of cultures grown on cellulose showed not only ..beta..-glucanase byt also ..beta..-xylanase activity. The latter could be attributed to an endo-1,4-..beta..-glucanase activity which had a low ..beta..-xylanase activity. 54 references.

• Research Organization: Universitaet Bransschweig, D-3300 Brunswick, Germany, F.R.
• OSTI ID: 5328232
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 51:4; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
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59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIODEGRADATION
CARBOHYDRATES
CELLULOSE
CHEMICAL REACTIONS
CULTURE MEDIA
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
GROWTH
HEMICELLULOSE
HYDROLASES
INHIBITION
MICROORGANISMS
O-GLYCOSYL HYDROLASES
OPTIMIZATION
ORGANIC COMPOUNDS
PH VALUE
POLYSACCHARIDES
SACCHARIDES
XYLANASE
XYLANS