Characterization of the binding of benzo(a)pyrene (BP) to a 4S cytosolic protein

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5325417

Houser, W H; Zielinski, R; Bresnick, E

The authors have recently reported on the partial purification of a 4S binding protein that interacts in a specific and saturable manner with (/sup 3/H)BP and other polycyclic aromatic hydrocarbons (PAH). They also reported that the 4S binding protein was able to interact in a specific and saturable manner with plasmids collectively containing the rat cytochrome P450c gene. The authors have examined further the (/sup 3/H)BP binding properties of the 4S protein. The specific (/sup 3/H)BP binding activity appears to be highest in 4 week old male rats and declines with age. In some animals the specific (/sup 3/H)BP binding activity is induced after pretreatment with either phenobarbital (BP) or isosafrole (IS) as evidenced by a 75% and 52% increase, respectively, over untreated controls. No apparent increase was observed after pretreatment of animals with 3-methylcholanthracene (3MC). The addition of a 200 fold excess of tetra-chlordibenzofuran (TCDBF) to the incubations did not displace (/sup 3/H)BP from the 4S protein. The addition of molybdate (10 mM) to isolation buffers, known to stabilize certain hormone receptors, did not alter the sedimentation coefficient or the specific binding activity of the 4S protein. The authors conclude that: (1) in the rat, the 4S protein appears to be distinct from the 8S protein reported in the mouse and that: (2) the 4S species regulates the PAH induced expression of AHH activity in the rat.

• Research Organization: Univ. of Nebraska Medical Center, Omaha
• OSTI ID: 5325417
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ANESTHETICS
ANIMALS
ANTHRACENE
ANTICONVULSANTS
AROMATICS
AZINES
BARBITURATES
BENZOFURANS
BENZOPYRENE
BIOCHEMICAL REACTION KINETICS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CONDENSED AROMATICS
DRUGS
FURANS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYPNOTICS AND SEDATIVES
KINETICS
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MOLYBDATES
MOLYBDENUM COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PHENOBARBITAL
PROTEINS
PURIFICATION
PYRIMIDINES
RATS
REACTION KINETICS
REFRACTORY METAL COMPOUNDS
RODENTS
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
VERTEBRATES