Interaction of the 4S polycyclic hydrocarbon-binding protein with the cytochrome P450c gene
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6060682
The 4S polycyclic hydrocarbon binding protein has been purified from rat liver and its properties examined. The protein was incubated with subclones from the P450c gene; it specifically interacted with a plasmid that contained the 5'-half of intron 1, exon 1 and 5'-flanking sequences. Exonuclease foot-printing after binding of the 4S protein to portions of the P450c gene showed protection at -200 and -400 bp from exon 1. The region -882 to +2545bp was constructed before a reporter, chloramphenicol acetyl transferase (CAT) gene in a plasmid that contained the SV40 ori, polyA signals, ampicillin resist gene. The P450c region contained promoter and putative regulatory sequences. The construct was transfected into rat hepatocytes, RL-PR-C and into rat hepatoma cells, H-4-11-E. After addition of 3-methylcholanthrene (3MC), CAT expression was induced. When the plasmid was constructed with the P450c fragment inverted, no CAT expression was seen. Deletion of -95 to -665 or from -238 to -660 bp eliminated the expression of CAT in response to 3MC. These experiments indicated the importance of this region in the induction of P450c by 3MC.
- Research Organization:
- Univ. of Nebraska Medical Center, Omaha
- OSTI ID:
- 6060682
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
FRACTIONATION
GENES
HYDROCARBONS
KINETICS
LIVER CELLS
MAMMALS
ORGANIC COMPOUNDS
PLASMIDS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
FRACTIONATION
GENES
HYDROCARBONS
KINETICS
LIVER CELLS
MAMMALS
ORGANIC COMPOUNDS
PLASMIDS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES