Hypothalamic norepinephrine turnover response to a single low protein, high carbohydrate meal in the male Wistar rat

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5321971

Raum, W; Glick, Z

A single meal stimulates norepinephrine turnover (NET) by approximately 4-fold in the brown adipose tissue (BAT). In this experiment the role of the hypothalamus in regulating this response was examined. NET was measured in the cortex (C), ventro-medial (VMH), and the lateral hypothalamus (LH). A total of 48 male Wistar rats (200 g body weight) were trained to eat during two feeding sessions per day. On the experimental day, one group (N = 24) was meal deprived and the other (N = 24) was given a low protein, high carbohydrate test meal for 2 hours. NET was determined by the synthesis inhibition method using alpha-methyltyrosine (AMT) injected within one hour after the meal. Norepinephrine (NE) content in each brain section was measured by radioimmunoassay at 4 time points (0, 1, 2, 3 hours) after AMT. The turnover rate (TR) was calculated as the slope of the decline in NE content over time (ng/mg protein/hr) following AMT. The fraction of the total pool of NE released/hr (k) was calculated by dividing the slope (TR) by the Y intercept (NE content at zero time). NET (TR) increased significantly (p < .05) in the VMH following a meal (9.36 +/- .67 vs 8.04 +/- .98 ng/hr; fed vs deprived). There was no change in TR in the C or LH, or in k in any brain section. The marked (4-fold) increase in BAT NET and minimal increase in VMH NET suggests that the meal has a direct effect on BAT with the VMH playing a secondary or modulatory role.

• Research Organization: UCLA Medical Center, Torrance
• OSTI ID: 5321971
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ADRENAL HORMONES
ANIMAL TISSUES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CONNECTIVE TISSUE
DRUGS
FOOD
HORMONES
HYPOTHALAMUS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SECRETION
STEROID HORMONES
SYMPATHOMIMETICS
TISSUES
TRACER TECHNIQUES
VERTEBRATES