Norepinephrine turnover in heart of the copper deficient rat

Journal Article · Wed Feb 01 00:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:7190220

Seidel, K E; Failla, M L; Rosebrough, R ^[1]

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1. Dept. of Agriculture, Beltsville, MD (USA)

Weaned male SD rats were fed a modified AIN-76A diet containing 62% sucrose and either 7 ppm (+Cu) or 0.5 ppm (-Cu) copper for 5 weeks. Dietary copper deprivation resulted in lower concentrations of copper in liver and serum and enlarged hearts. Tissue levels of norepinephrine (NE) and dopamine (DPA) were quantified by HPLC using electrochemical detection. Cardiac concentration of NE and DPA and 26% lower and 63% higher, respectively, in -Cu rats than in +Cu controls. Altered cardiac levels of NE and DPA in -Cu rats were also evident after overnight fasting, a stress that depresses SNS activity. NE turnover was investigated after inhibition of tyrosine hydroxylase by injection of {alpha}-methyl-p-tyrosine methyl ester (250 mg/kg). The fractional rate of NE turnover in the heart was 4.6%/hour for rats fed -Cu and +Cu diets. Calculated NE turnover was greater in heart of +Cu rats than -Cu rats (26 vs. 19 ng/g/hr). NE and DPA concentration in brain, pancreas, and spleen were not affected by dietary copper. These data suggest that synthesis of NE in cardiac nerve endings of the weaned rats sensitive to dietary copper deficiency.

• OSTI ID: 7190220
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Vol. 3:4; ISSN 0892-6638
• Country of Publication: United States
• Language: English

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