Copper-deficient mice have higher cardiac norepinephrine turnover

Journal Article · Wed Feb 01 04:00:00 EST 1989 · FASEB Journal (Federation of American Societies for Experimental Biology); (USA)

OSTI ID:6832620

Gross, A M; Prohaska, J R ^[1]

---

1. Univ. of Minnesota, Duluth (USA)

Male Swiss albino mice were studied at 6 weeks of age. Their dams were fed a copper-deficient diet (modified AIN-76A) starting 4 days after birth and given deionized water (-Cu) or water with CuSO{sub 4} added (+Cu) (20 {mu}g Cu/ml). When 3 weeks of age mice were weaned and housed in stainless steel cages on the respective treatment of their dams. Turnover of norepinephrine (NE) was studied in 8 experiments using 2 separate techniques. The first procedure used {alpha}-methyl-p-tyrosine methyl ester (300 mg/kg i.p.) to inhibit tyrosine hydroxlase activity. The loss of residual NE was determined by HPLC with electrochemical detection. Regression lines were constructed and fractional turnover (%/h) and calculated turnover (ng/g/h) were determined for heart, cerebellum and adrenal gland. In 4 experiments loss of NE in cerebellum of -Cu ad +Cu mice was equivalent. Loss of NE from adrenal gland could not be detected in the 8 h time course. Loss of NE, both fractional turnover and calculated turnover, from heart of -Cu mice was 4-5 fold higher compared to +Cu controls. A second method using m- hydroxybenzylhydrazine (NSD-1015) (100 mg/kg i.p.), which inhibits aromatic amino acid decarboxylase, confirmed the results. For all 4 experiments the cardiac accumulation of L-DOPA (measured by HPLC) was faster in -Cu mice compared to controls. The higher turnover rate of NE in heart and perhaps other sympathetic nerves may contribute to the higher urinary NE output observed previously.

• OSTI ID: 6832620
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (USA), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (USA) Vol. 3:4; ISSN FAJOE; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL GLANDS
ADRENAL HORMONES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
CEREBELLUM
COPPER
DRUGS
ELEMENTS
ENDOCRINE GLANDS
GLANDS
HEART
KINETICS
MAMMALS
METALS
MICE
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
NUTRITIONAL DEFICIENCY
ORGANS
REACTION KINETICS
RODENTS
SYMPATHOMIMETICS
TRANSITION ELEMENTS
VERTEBRATES