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Inhibition by complex organic mixtures of benzo(a)pyrene- and diethylnitrosamine initiation of preneoplastic changes in rat liver

Technical Report ·
OSTI ID:5321955
Previous studies using the mouse skin initiation-promotion model have shown that initiating activities of pure carvinogens are decreased when they are applied simultaneously with complex coal-derived mixtures. In the present study the number of altered hepatocyte foci which stained for gamma glutamyl transpeptidase (GGT) were determined in rats that were neonatally treated with an initiator, followed by promotion with dietary phenobarbitol for 8 weeks after weaning. Initiators were either benzo(a)pyrene (BaP), diethylnitrosamine (DEN), or complex coal-derived mixtures. Although the compled organic mixtures contained known carcinogens, their initiating activities were low at the doses used. Co-administration of complex, coal-derived mixtures with BaP or DEN decreased the response to both carcinogens. Together with data from studies with the skin initiation-promotion model, the results suggest that tumorigenic activities of coal-derived complex organic mixtures are less than predicted from the content of carcinogenic components. 20 refs., 2 tabs.
Research Organization:
Pacific Northwest Lab., Richland, WA (USA). Biology and Chemistry Dept.; Argonne National Lab., IL (USA). Biological and Medical Research Div.
DOE Contract Number:
AC06-76RL01830
OSTI ID:
5321955
Report Number(s):
PNL-SA-13204; ON: DE86015539
Country of Publication:
United States
Language:
English