Effect of fluoride on oxidative phosphorylation and dehydrogenase systems in higher plants
Studies were conducted on the in vitro effect of fluoride on the succinic oxidase system utilizing mitochondria obtained from cauliflower. Methods are described for extracting mitochondria and determining maximum activities and inhibition using various treatments. Experiments were conducted in the absence of fluoride and using various concentrations of phosphate to determine the effect of phosphate alone on the succinic 2.6-dichlorophenolindophenol reductase component of the succinic oxidase system. These studies showed that phosphate had only slight effects on succinic 2.6-dichlorophenolindophenol reductase. An oxidative phosphorylation study was conducted to determine effects of fluoride on phosphorus esterification and oxygen uptake. It was found in general that respiration and phosphorylation were inhibited. The effects of phosphate in the presence of sodium fluoride were studied using various concentrations of phosphate. It was found that the middle range of phosphate concentrations used in these studies inhibited oxidation to a greater degree than at the extremes. An increased oxygen uptake at higher concentrations was observed. In vivo effects of fluoride on TCA cycle dehydrogenase enzymes of Pelargonium zonale were studied using p-nitro-blue-tetrazoleum chloride. Plants were exposed to 17 ppb HF and enzyme activities were compared to control plants. Leaves of control plants were also incubated in 5 X 10/sup -3/ M NaF. Injury observed in both fumigation and solution experiments was similar. Leaf tissue subjected to HF or NaF had less succinic p-nitro-blue-tetrazoleum chloride reductase activity than did the control tissue. Other Kreb's acid dehydrogenases were not inhibited by fluoride concentrations used in these experiments.
- Research Organization:
- Utah State Univ., Logan (USA)
- OSTI ID:
- 5319116
- Country of Publication:
- United States
- Language:
- English
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54 ENVIRONMENTAL SCIENCES
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
ALKALI METAL COMPOUNDS
BIOLOGICAL EFFECTS
BRASSICA
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELECTRON TRANSFER
ELEMENTS
ENZYME ACTIVITY
ENZYMES
FLUORIDES
FLUORINE COMPOUNDS
FOOD
HALIDES
HALOGEN COMPOUNDS
HYDROFLUORIC ACID
HYDROGEN COMPOUNDS
IN VITRO
INHIBITION
INORGANIC ACIDS
KREBS CYCLE
LEAVES
MITOCHONDRIA
NONMETALS
ORGANOIDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS
PHOSPHORUS COMPOUNDS
PHOSPHORYLATION
PLANTS
POLLUTION
RESPIRATION
RESPONSE MODIFYING FACTORS
SODIUM COMPOUNDS
SODIUM FLUORIDES
VEGETABLES