Histochemical investigations on the in vivo effects of fluoride on tricarboxylic acid cycle dehydrogenases from Pelargonium zonale. Part II
In vivo effects of fluoride on tricarboxylic acid (TCA) cycle dehydrogenase enzymes of Pelargonium zonale were studied using p-nitro blue tetrazoleum chloride. Plants were exposed to 17 ppb HF, and enzyme activities in treated plants were compared to those in controls. Leaves of control plants were incubated in 5 x 10/sup -3/ M sodium fluoride. Injuries observed in fumigation and solution experiments were similar. Leaf tissue subjected to HF or sodium fluoride evidenced less succinic p-nitro blue tetrazoleum reductase activity than did control tissue. Other TCA cycle dehydrogenase enzymes were not observably affected by the fluoride concentrations used in these experiments. Excised leaves cultured in 5 x 10/sup -3/ M sodium fluoride exhibited less succinic p-nitro blue tetrazoleum reductase activity after 24 hr than did leaves cultured in 5 x 10/sup -3/ M sodium chloride. 8 references, 8 figures.
- Research Organization:
- Humboldt State College, Arcata, CA
- OSTI ID:
- 5434576
- Journal Information:
- J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 15:4; ISSN JHCYA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
CARBOXYLIC ACIDS
ENZYME ACTIVITY
ENZYMES
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
HYDROFLUORIC ACID
HYDROGEN COMPOUNDS
IN VIVO
INJURIES
INORGANIC ACIDS
LEAVES
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORNAMENTAL PLANTS
OXIDOREDUCTASES
PLANTS
SODIUM COMPOUNDS
SODIUM FLUORIDES
TOXICITY