Effects of fluoride on respiration, oxidative phosphorylation, mitochondrial ATPase activity, and mitochondrial permeability
Fluoride was found to inhibit succinic, malic, and NADH dehydrogenase and to partially uncouple the phosphorylation associated with these enzymes. Etiolated soybean hypocotyl sections were treated with fluoride. This treatment reduced the succinic dehydrogenase activity and increased the malic dehydrogenase activity of the isolated mitochondria. The ADP/O ratios and respiratory control ratios were reduced for both substrates. The separate and combined effects of fluoride and dinitrophenol on ATPase activity of isolated mitochondria were studied. Dinitrophenol stimulated ATPase activity, and fluoride eliminated the dinitrophenol stimulation. Fumigation of soybean leaf tissue with hydrogen fluoride resulted in an initial stimulation of respiration. Mitochondria extracted from this tissue exhibited increased respiration, increased ATPase activity, and very low P/O ratios. With a longer period of fumigation, the respiratory rate of the tissue was inhibited. Mitochondrial respiration, ATPase activity, and phosphorylation were also inhibited. The effect of fluoride on mitochondrial swelling and release of proteins were examined. The presence of fluoride caused a more rapid osmotically-induced swelling and resulted in an accelerated release of proteins from the mitochondria.
- Research Organization:
- Utah State Univ., Logan (USA)
- OSTI ID:
- 5319300
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
AROMATICS
ATP-ASE
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DINITROPHENOL
ENZYME ACTIVITY
ENZYMES
FLUORIDES
FLUORINE COMPOUNDS
GLYCINE HISPIDA
HALIDES
HALOGEN COMPOUNDS
HYDROFLUORIC ACID
HYDROGEN COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
INHIBITION
INORGANIC ACIDS
LEGUMINOSAE
MITOCHONDRIA
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PERMEABILITY
PHENOLS
PHOSPHOHYDROLASES
PHOSPHORYLATION
PLANTS
RESPIRATION
STIMULATION