Improved monoclonal antibodies to halodeoxyuridine

Patent · Tue Oct 18 04:00:00 EDT 1983

OSTI ID:5313860

Vanderlaan, M; Dolbeare, F A; Gray, J W; Thomas, C B

The development, method of production, characterization and methods of use of two hybridomas, CIdU-1 (ATCC Accession No. HB-8321) and CIdU-2 (ATCC Accession No. HB-8320), are described. These secrete IgG/sub 1/(K) immunoglobulins that react with halodeoxyuridine (HdU or halodU) such as bromo, chloro, fluoro and iodo deoxyuridine (BrdU, CldU, FdU and IdU), whether these are free in solution or incorporated into single stranded DNA in whole cells. The antibodies do not react with naturally occurring free nucleic acids or with deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) polymers. These antibodies are suitable for use in enzyme immunoassays for free CldU, FdU, IdU and BrdU and for detecting cells with these nucleotides incorporated into them. The monoclonal antibodies are useful in the detection of the sensitivity of tumor cells to specific chemotherapeutic agents, in the measurement of the rate of cellular DNA synthesis, in the measurement of the rate of proliferation of normal and malignant cells and in the detection of HPRT deficiency in cells. 1 tab.

• Research Organization: Lawrence Livermore National Lab., CA (USA)
• DOE Contract Number: W-7405-ENG-48
• Assignee: Dept. of Energy
• Patent Number(s): None
• Application Number: ON: DE85017764
• OSTI ID: 5313860
• Country of Publication: United States
• Language: English

Related Subjects

550600* -- Medicine
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBODIES
ANTIMETABOLITES
ANTIMICROBIAL AGENTS
AZINES
BIOASSAY
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BROMOURACILS
BUDR
CELL FLOW SYSTEMS
CHO CELLS
DIAGNOSTIC USES
DNA REPAIR
DRUGS
ENZYME IMMUNOASSAY
ENZYMES
FLUOROURACILS
FUDR
GLYCOSYL TRANSFERASES
HETEROCYCLIC COMPOUNDS
HYBRIDOMAS
HYDROXY COMPOUNDS
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
IODODEOXYURIDINE
IODOURACILS
MONOCLONAL ANTIBODIES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSYL TRANSFERASES
PYRIMIDINES
RADIOSENSITIZERS
RECOVERY
REPAIR
RIBOSIDES
TRANSFERASES
URACILS
USES