Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · J. Bacteriol.; (United States)

OSTI ID:5313069

Wang, Y; Mahler, I; Levinson, H S; Halvorson, H O

A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to HgCl/sub 2/ and to phenylmercury acetate (PMA) was expressed. Shortening the cloned fragment to 3.8 kb abolished resistance to PMA but not to HgCl/sub 2/. In Bacillus subtilis, the 3.8-kb fragment produced mercuric reductase constitutively but did not produce resistance to HgCl/sub 2/ or to PMA.

• Research Organization: Brandeis Univ., Waltham, MA (USA)
• OSTI ID: 5313069
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 169:10; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACILLUS
BACTERIA
CELL CONSTITUENTS
CHLORIDES
CHLORINE COMPOUNDS
CLONING
DETOXIFICATION
DNA
DNA-CLONING
ELEMENTS
ENZYMES
ESCHERICHIA COLI
GENETIC MAPPING
HALIDES
HALOGEN COMPOUNDS
MAPPING
MERCURY
MERCURY CHLORIDES
MERCURY COMPOUNDS
MERCURY HALIDES
METALS
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC MERCURY COMPOUNDS
OXIDOREDUCTASES
PLASMIDS
RECOMBINANT DNA
SENSITIVITY