Molecular cloning of an endoglucanase gene from an alkalophilic bacillus sp. and its expression in escherichia coli
One of the cellulase genes from alkalophilic Bacillus sp. strain N-4 was cloned in pBR322. A recombinant plasmid, pYBC107, expressing carboxymethyl cellulase (CMCase) was isolated, and the size of the cloned HindIII fragment was found to be 5.5 kilobases. The restriction map of pYBC107 showed a different pattern from those of pNKI and pNKII. When the HindIII fragment from pYBC107 was subcloned into pYEJ001, there was a 3.8-fold increase in CMCase activity over that observed with pYBC107. Plasmid pYBC108 constructed by treatment of pYBC107 with HindIII and ECORI expressed the CMCase activity, although to a limited extend. To verify the originality of cloned pYBC107 from Bacillus sp;, the authors analyzed the restriction digest by Southern blotting.
- Research Organization:
- Yonsei Univ., Seoul (Korea)
- OSTI ID:
- 7050382
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 53:11; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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