Functional and morphologic changes caused by acute ozone exposure in the isolated and perfused rat lung

Journal Article · Wed Apr 01 04:00:00 EST 1992 · American Review of Respiratory Disease (New York); (United States)

DOI:https://doi.org/10.1164/ajrccm/145.4_Pt_1.882· OSTI ID:5308824

Pino, M V; McDonald, R J; Berry, J D; Joad, J P; Tarkington, B K; Hyde, D M ^[1]

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1. California Primate Research Center, University of California, Davis (United States)

Ozone has been shown to increase airway resistance and/or airway reactivity in vivo in animals and humans. Because of the complexities inherent in studying this phenomenon in whole animals, we developed a model of ozone-induced effects on airway physiology using the isolated perfused rat lung. Rat lungs were suspended in an airtight chamber and perfused via the pulmonary circulation with a modified Krebs-Henseleit buffer containing 4.5% bovine albumin. Ventilation of the lungs was achieved by generating a fluctuating negative pressure within the chamber (-2 to -7 cm H2O) at a rate of 60 breaths/min. The lungs were ventilated with humidified 95% air and 5% CO2 alone (control condition) or mixed with ozone at 1.0 or 2.0 ppm. Transpulmonary pressure, flow rate, and tidal volume were recorded at 0, 1, 2, and 3 hours, and pulmonary resistance (RL) and dynamic compliance (Cdyn) were calculated. There was no significant difference in lung weight/total body weight ratios between the three groups at the end of the 3-h period. RL increased and Cdyn decreased in a time- and dose-dependent manner with ozone exposure. The percent increase above baseline in RL +/- SEM at 3 h was 9.4 +/- 4.1% for control lungs, 21.0 +/- 3.2% for 1.0 ppm ozone-exposed lungs, and 63.6 +/- 13.5% for 2.0 ppm ozone-exposed lungs. The percent decrease below baseline in Cdyn +/- SEM at 3 h was 27.4 +/- 2.1% for control lungs, 37.1 +/- 2.7% for 1.0 ppm ozone-exposed lungs, and 55.2 +/- 7.3% for 2.0 ppm ozone-exposed lungs.(ABSTRACT TRUNCATED AT 250 WORDS)

• OSTI ID: 5308824
• Journal Information: American Review of Respiratory Disease (New York); (United States), Journal Name: American Review of Respiratory Disease (New York); (United States) Vol. 145:4 Pt 1; ISSN ARDSB; ISSN 0003-0805
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ANIMALS
BODY
DOSE-RESPONSE RELATIONSHIPS
LUNGS
MAMMALS
MORPHOLOGICAL CHANGES
ORGANS
OZONE
PERFUSED ORGANS
RATS
RESPIRATORY SYSTEM
RODENTS
TIME DEPENDENCE
TOXICITY
VERTEBRATES