Red cell proteins. I. Two-dimensional mapping of human erythrocyte lysate proteins
Human erythrocyte lysate proteins were resolved into over 250 discrete spots by two-dimensional electrophoresis using isoelectric focusing in the first dimension and electrophoresis in the presence of sodium dodecyl sulfate (SDS) in the second. The overwhelming excess of hemoglobin has made such analyses difficult in the past. However, with the ISO-DALT two-dimensional electrophoresis system, large numbers of red cell proteins can be mapped in the presence of hemoglobin. When hemoglobin and several other major proteins are removed by adsorption to DEAE-cellulose, additional minor components are seen, giving a total of over 275. With the use of purified preparations, the map positions of five red cell enzymes or their subunits were determined: pyruvate kinase, catalase, glucose-6-phosphate dehydrogenase, hypoxanthine phosphoribosyltransferase, and carbonic anhydrase. The mapping techniques described complement and extend those traditionally used to find human red cell protein variants.
- Research Organization:
- Argonne National Lab., IL
- OSTI ID:
- 5308262
- Journal Information:
- Blood; (United States), Journal Name: Blood; (United States) Vol. 53:6; ISSN BLOOA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BIOLOGICAL VARIABILITY
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBONIC ANHYDRASE
CARBOXYLIC ACIDS
CATALASE
CHEMICAL ANALYSIS
CHEMISTRY
DEHYDROGENASES
ELECTROPHORESIS
ENZYMES
ERYTHROCYTES
GLOBIN
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
LYASES
MAMMALS
MAN
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PHOSPHOTRANSFERASES
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
QUALITATIVE CHEMICAL ANALYSIS
TRANSFERASES
TWO-DIMENSIONAL CALCULATIONS
VERTEBRATES