Quantitative determination of native proteins in individual human erythrocytes by capillary zone electrophoresis with laser-induced fluorescence detection
- Ames Lab., IA (United States) Iowa State Univ., Ames (United States)
Intracellular fluid within single human erythrocytes is analyzed by capillary electrophoresis with laser-excited native protein fluorescence. Good signal-to-noise is achieved, allowing even minor components to be quantified. Non-Gaussian distributions were found for total protein, fraction carbonic anhydrase, fraction hemoglobin A[sub 0], and an unidentified component. Variations among a group of 29 cells for each quantity are as much as 1 order of magnitude, even though erythrocytes are known to be fairly homogeneous in size distribution. Variations in fraction hemoglobin A[sub 0] reflect differences in in vitro oxidation rates to methemoglobin. A positive correlation was observed between carbonic anhydrase and hemoglobin A[sub 0] for individual cells. This is consistent with the presence of erythrocytes of different ages within the population, with the older cells being less capable of maintaining enzyme activity and preventing oxidative damage. 35 refs., 10 figs., 1 tab.
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6792422
- Journal Information:
- Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 64:23; ISSN ANCHAM; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
Similar Records
Indirect fluorescence detection of native amino acids in capillary zone electrophoresis
Direct analysis of cellular proteins by capillary electrophoresis FTICR MS
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AGING
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON-OXYGEN LYASES
CARBONIC ANHYDRASE
CARBOXYLIC ACIDS
CHEMICAL COMPOSITION
DATA
ELECTROPHORESIS
EMISSION SPECTROSCOPY
ENZYMES
ERYTHROCYTES
EXPERIMENTAL DATA
FLUORESCENCE SPECTROSCOPY
GLOBINS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDRO-LYASES
INFORMATION
LASER SPECTROSCOPY
LYASES
MATERIALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
SPECTROSCOPY