Cellular kinetics in a patient with Sezary syndrome

Journal Article · Thu Jan 01 04:00:00 EST 1981 · Am. J. Hematol.; (United States)

DOI:https://doi.org/10.1002/ajh.2830100403· OSTI ID:5307515

Chandra, P; Chanana, A D; Chikkappa, G; Cronkite, E P

Cellular kinetics and proliferation of Sezary cells (SC) were studied in a 48-year-old woman with Sezary syndrome (SS). In vitro flash labeling indices of peripheral blood (PB) SC were studied by labeling with tritiated thymidine (/sup 3/HTdR) and tritiated cytidine (/sup 3/HCdR). Intradermal SC were labeled in vivo by local injection of /sup 3/HTdR followed by skin biopsies of the injected sites. Traffic patterns of DNA labeled PB SC were studied by intravenous /sup 3/HTdR followed by sampling of PB, skin, lymph node (LN), and bone marrow (BM). Fluxes of PB SC in various tissues were investigated by autotransfusion of /sup 3/HCdR labeled PB SC followed by serial sampling of PB, LN, BM, and skin. In vitro response of PB SC to phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed (PWM) were also investigated. The results from these studies in this patient indicate that 1) proliferation of SC was primarily in the skin, 2) there was a negligible flux of SC from blood into skin, LN, and BM, and 3) the mitogenic response of PB mononuclear cells (mostly SC) to PHA, PWM, and Con A was poor.

• Research Organization: Brookhaven National Lab., Upton, NY
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 5307515
• Journal Information: Am. J. Hematol.; (United States), Journal Name: Am. J. Hematol.; (United States) Vol. 10; ISSN AJHED
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BIOPSY
BODY
BONE MARROW
CELL PROLIFERATION
DIAGNOSTIC TECHNIQUES
DISEASES
DNA
FEMALES
HEMATOPOIETIC SYSTEM
KINETICS
LABELLED COMPOUNDS
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
MAN
METABOLIC DISEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATIENTS
PRIMATES
SKIN
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES
WOMEN