DNA segment containing C/sub. beta. 1/, a gene for the constant region of the. beta. chain of the T-cell antigen receptor, was inserted into chromosome 6 in cells from one patients with human T-cell leukemia

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.12.4264· OSTI ID:5303141

Ino, T; Kurosawa, Y; Yoshida, M C; Hirano, M

DNA rearrangements that occurred in the vicinity of T-cell antigen receptor ..beta..-chain gene clusters residing on chromosome 7 were examined in human T-cell acute lymphoblastic leukemia cells. In one patient, it was observed that, for the T-cell receptor ..beta..-chain genes, a D/sub ..beta.. 1/-J/sub ..beta..2.3/ (where D is diversity and J is joining) junction was found on one chromosome, while the other chromosome kept the germ-line configuration. If this D/sub ..beta../-J/sub ..beta../ junction was formed by the customary deletion mechanism, the C/sub ..beta..1/ gene (where C is constant) located between the D/sub ..beta..1/ and J/sub BETA2.3/ loci should have disappeared from this chromosome. The C/sub ..beta..1/ gene indeed was absent from the rearranged chromosome 7, but it was found on chromosome 6 as an inserted segment. The implications of the observations are discussed.

• Research Organization: Fujita-Gakuen Health Univ., Aichi, Japan
• OSTI ID: 5303141
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:12
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
DNA
AUTORADIOGRAPHY
TUMOR CELLS
GENE RECOMBINATION
ANTIGENS
CHROMOSOMAL ABERRATIONS
DNA SEQUENCING
ELECTROPHORESIS
GENES
HETEROCHROMOSOMES
LEUKEMIA
LYMPHOCYTES
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CHROMOSOMES
CONNECTIVE TISSUE CELLS
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LEUKOCYTES
MATERIALS
MEMBRANE PROTEINS
MUTATIONS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
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