Neutrophil glycoprotein Mo1 is an integral membrane protein of plasma membranes and specific granules

Journal Article · Tue Dec 01 04:00:00 EST 1987 · J. Immunol.; (United States)

OSTI ID:5301820

Stevenson, K B; Nauseef, W M; Clark, R A

The glucoprotein Mo1 has previously been demonstrated to be on the cell surface and in the specific granule fraction of neutrophils and to be translocated to the cell surface during degranulation. It is not known, however, whether Mo1 is an integral membrane protein or a soluble, intragranular constituent loosely associated with the specific granule membrane. Purified neutrophils were disrupted by nitrogen cavitation and separated on Percoll density gradients into four fractions enriched for azurophilic granules, specific granules, plasma membrane, and cytosol, respectively. The glycoproteins in these fractions were labeled with /sup 3/H-borohydride reduction, extracted with Triton X-114, and immunoprecipitated with 60.3, an anti-Mo1 monoclonal antibody. Mo1 was detected only in the specific granule and plasma membrane fractions and partitioned exclusively into the detergent-rich fraction consistent with Mo1 being an integral membrane protein. In addition, treatment of specific granule membranes with a high salt, high urea buffer to remove adsorbed or peripheral proteins failed to dissociate Mo1. These data support the hypothesis that Mo1 is an integral membrane protein of plasma and specific granule membranes in human neutrophils.

• Research Organization: Univ. of Iowa College of Medicine, Iowa City
• OSTI ID: 5301820
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 139:11; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGENS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BOROHYDRIDES
BORON COMPOUNDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
ELECTROPHORESIS
GLUCOPROTEINS
HYDROGEN COMPOUNDS
LABELLED COMPOUNDS
LEUKOCYTES
MATERIALS
MEMBRANES
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NEUTROPHILS
ORGANIC COMPOUNDS
PRECIPITATION
PROTEINS
SACCHARIDES
SEDIMENTATION
SEPARATION PROCESSES
TRITIUM COMPOUNDS