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Role for membrane fusion in the activation of the respiratory burst in human neutrophils

Thesis/Dissertation ·
OSTI ID:5348994
Components of the respiratory burst oxidase reside in intracellular membranes of the tertiary granules in resting cells, yet oxidase activity in the activated cells occurs at the neutrophil surface. The role of degranulation in activation of the neutrophil respiratory burst was therefore investigated. Surface labeling experiments were carried out on resting and activated neutrophils using three impermeant labeling methods. Activated neutrophils labeled with (/sup 35/S) diazobenzene sulfonic acid showed a fourfold higher specific radioactivity than resting neutrophils. Similar results were obtained with the pyridoxal phosphate/borotritide labeling method. On the other hand, little difference in labeling was seen using the periodate/borotritide method which detects the carbohydrate of glycoproteins. These results suggest that either a large amount of protein, or a highly reactive protein becomes exposed upon activation. Resting, activated, and enucleated cells were labeled using the (/sup 125/I) lactoperoxidase method, then subjected to polyacrylamide gel electrophoresis. Autoradiograms of these gels showed that two proteins of about 75 and 45 kD, are labeled at the external surface of enucleated and activated cells but not resting cells.
Research Organization:
Dartmouth Coll., Hanover, NH (USA)
OSTI ID:
5348994
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLING
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
MEMBRANES
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
RADIOISOTOPES
RESPIRATION
SULFONIC ACIDS
SULFUR 35
SULFUR ISOTOPES